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| Registros recuperados: 92 | |
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| Boudry, Pierre; Chatain, Beatrice; Naciri-graven, Yamama; Lemaire, Christophe; Gerard, Andre. |
| In France, marine fish and shellfish farming is based mainly on the breeding of wild species, whose natural populations are traditionally exploited. Research programs are conducted by IFREMER, in co-operation with INRA and Universities. French research programs for the genetical improvement of marine fish and shellfish concern primarily seabass (Dicentrarchus labrax) and oysters (Ostrea edulis and Crassostrea gigas). The following programs are developed: The knowledge of wild and farmed populations. The most recent prodjects involve the highly variable microsatellite markers. Seven markers are presently available for seabass and the work is now initiated on turbot (Scophthalmus maximus). In molluscs, microsatellite markers have been developed for the... |
| Tipo: Text |
Palavras-chave: Selection; Triploid; Shellfish; Fish; Genetic. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/1996/acte-3206.pdf |
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| Peruzzi, Stefano; Chatain, Beatrice. |
| The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0-1 degrees C for 15-20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm(-2)) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was... |
| Tipo: Text |
Palavras-chave: Parental variability; Pressure and cold shocks; Dicentrarchus labrax; Sea bass; Triploidy; Meiotic gynogenesis. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-507.pdf |
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| Tamazouzt, Lakhdar; Chatain, Beatrice; Fontaine, Pascal. |
| The influence of interaction between light intensity and tank wall colours on survival and growth of perch larvae (Perca fluviatilis L.) was investigated for 15 days after hatching. Three light intensities (250, 400 and 800 Ix) and four wall colours (black, dark grey, light grey and white) were used. At the end of the experiment, survival rates ranged between 1% and 26%, and varied according to the different treatments (P = 0.0001; df = 11). A global effect of light intensity was detected(P = 0.0001; df = 2) and the highest survival rate was observed under 250 Ix. Survival was also influenced by the tank wall colour(P = 0.0001; df = 3): light grey showed the best result (17%). The greatest growth in weight and length was observed in tanks with light grey... |
| Tipo: Text |
Palavras-chave: Growth; Survival; Light level; Tank colour; Larvae; Eurasian perch. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-505.pdf |
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| Bahri Sfar, Lilia; Lemaire, Christophe; Chatain, Beatrice; Divanach, Pascal; Ben Hassine, Oum Kalthoum; Bonhomme, Francois. |
| A phylogeographic analysis based on 6 polymorphic microsatellites was performed on 15 sea bass samples. five from Western Mediterranean, seven from Eastern Mediterranean and three coming from French aquaculture stocks. Among the eastern samples, three did not cluster according to their geographic origin but rather with the occidental group. Furthermore. a somewhat lower allelic diversity was observed within these particular samples, indicating that they originated from a limited number of progenitors of foreign origin. Among the aquaculture stocks, only one revealed a significant reduction of genetic variability, indicating that these stocks are largely outbred and open to wild fishes. The fact that the use of occidental fingerlings to seed broodstocks in... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Farmed populations; Escapees; Molecular marker. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-410.pdf |
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| Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice. |
| The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable... |
| Tipo: Text |
Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf |
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| Chatain, Beatrice; Poline, Olivier. |
| Objectives : Presently, most of marine fish farmers around the Mediterranean basin grow genetically wild fish strains, which have not been genetically improved for culture performance (growth, appearance, disease resistance etc.). It is inevitable that the present gap for genetically improved strains of marine fish, and their reliable supply, will be filled. It will ultimately dominate the competitive component of marine culture industries once they evolve into commodity-style agribusiness (with fish prices being set at the lowest possible cost of production and the best quality). Those countries, which can competitively develop and supply the improved strains, will enjoy the benefit (perhaps survival) for their local industries. They will avoid... |
| Tipo: Text |
Palavras-chave: Pathogen free; Genetic origin; Sperm; Cryopreservation; Sea bass. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/rapport-6494.pdf |
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| Peruzzi, Stefano; Chatain, Beatrice; Menu, Bruno. |
| The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant... |
| Tipo: Text |
Palavras-chave: Anguilla anguilla; Liza ramada; Sparus aurata; Dicentrarchus labrax; Teleosts; DNA content. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-411.pdf |
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| Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert. |
| The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology. |
| Ano: 2005 |
URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf |
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| Peruzzi, Stefano; Westgaard, Jon-ivar; Chatain, Beatrice. |
| Although the actiology of swimbladder inflation anomalies in important aquaculture species such as European sea bass D. labrax is not fully determined, culture conditions are commonly suggested as main contributory factors. Little information is available on whether swimbladder inflation has a genetic basis for its expression too. In this work, 24 full-sibling sea bass families from a 4 dams x 6 sires factorial crossing were reared under communal conditions. The larvae developing normal and abnormal (uninflated or hyper-inflated) swimbladders were genotyped at four microsatellite loci, Labrax-3, Labrax-13, Labrax-17, Labrax29, and allocated to the individual breeders. Out of 273 offspring, 97% could be assigned to a single parental pair. The genotype and... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Genetics; Anomalies; Swimbladder. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2590.pdf |
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| Vandeputte, Marc; Dupont Nivet, M; Chavanne, H; Chatain, Beatrice. |
| Polygenic sex determination, although suspected in several species, is thought to be evolutionarily unstable and has been proven in very few cases. In the European sea bass, temperature is known to influence the sex ratio. We set up a factorial mating, producing 5.893 individuals from 253 full-sib families, all reared in a single batch to avoid any between-families environmental effects. The proportion of females in the offspring was 18.3%, with a large variation between families. Interpreting sex as a threshold trait, the heritability estimate was 0.62 +/- 0.12. The observed distribution of family sex ratios was in accordance with a polygenic model or with a four-sex-factors system with environmental variance and could not be explained by any genetic... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Fish; Growth; Heritability; Sex ratio. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3526.pdf |
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| Dupont Nivet, M; Vandeputte, Marc; Vergnet, Alain; Merdy, O; Haffray, P; Chavanne, H; Chatain, Beatrice. |
| 253 full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank during 14 months, then 7000 of them were dispatched in four farms to different locations (France, Israel, Italy, Portugal) representing a wide variety of environmental conditions. Around 400 g mean weight, 1177 to 1667 fish in each site were weighed and length was measured. Condition factor (K) was calculated. Pedigrees were redrawn a posteriori using microsatellites markers: parental origin could be retraced for 99.2% of fish. Due to a high incidence of deformities, the useful sample size was reduced to 491-670 fish per site. Maternal effects were small. Using a simple animal model, heritability... |
| Tipo: Text |
Palavras-chave: Microsatellites; GxE interactions; Heritability; Growth; Seabass; Dicentrarchus labrax. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4404.pdf |
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| Sauzet, S; Besseau, L; Perez, P; Coves, Denis; Chatain, Beatrice; Peyric, E; Boeuf, G; Munoz Cueto, J; Falcon, J. |
| Melatonin contributes to synchronizing behaviors and physiological functions to daily and seasonal rhythm in fish. However, no coherent vision emerges because the effects vary with the species, sex, moment of the year or sexual cycle. And, scarce information is available concerning the melatonin age, receptors, which is crucial to our understanding of the role melatonin plays. We report here the full length cloning of three different melatonin receptor subtypes in the sea bass Dicentrarchus labrax, belonging, respectively, to the MT1, MT2 and Mellc subtypes. MT1, the most abundantly expressed, was detected in the central nervous system, retina, and gills. MT2 was detected in the pituitary gland, blood cells and, to a lesser extend, in the optic tectum,... |
| Tipo: Text |
Palavras-chave: Retina; Melatonin receptors; Sea bass. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4513.pdf |
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| Vandeputte, Marc; Dupont Nivet, M; Haffray, P; Chavanne, H; Cenadelli, S; Parati, K; Vidal, Marie-odile; Vergnet, Alain; Chatain, Beatrice. |
| Selective breeding of European sea bass (Dicentrarchus labrax) receives a growing interest, as the estimated heritability of growth is medium to high. In this study, we compared the offspring of four groups of sea bass sires, mated with the same wild dams: wild (W), first generation of domestication (D), first generation of mass selection for length (M), first generation of PROSPER-like selection for length (P). The comparison was done both in replicated tanks (separate rearing) and in mixed tanks (mixed rearing) where sire origins were recovered by genotyping of eight microsatellite markers. Weight, length and growth rate were measured from day 238 post-fertilization (69 g mean weight) to day 611 post-fertilization (390 g mean weight). Both in mixed and... |
| Tipo: Text |
Palavras-chave: Nephrocalcinosis; Competition; Microsatellites; Parentage assignment; Realized heritability; Selection response; Growth; Selective breeding; Dicentrarchus labrax; European sea bass. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-5303.pdf |
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| Millot, Sandie; Begout, Marie-laure; Chatain, Beatrice. |
| Differences in bold and shy personality on sea bass Dicentrarchus labrax were investigated between a population (wild) produced from wild-brood fish and a population (selected) produced from selected-brood fish. During the experiment (112 days), fish were reared under self-feeding condition to characterize the feeding behaviour of each individual fish. Three risk-taking tests (T1, T2 and T3 of 24 h with day-night alternation) were carried out at > 1 month intervals on 180 fish of each strain in order to monitor D. labrax behaviour over time and in relation to the light:dark period. A risk-taking score was evaluated via a preference choice between a safe zone (without food) and a risky zone (potentially with food) by recording the number and the duration... |
| Tipo: Text |
Palavras-chave: Learning; Habituation; Domestication; Choice test; Boldness. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7313.pdf |
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| Registros recuperados: 92 | |
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